|
|
In vitro 3D organization of the mammalian testes
Zahradníková, Hana ; Tlapáková, Tereza (advisor) ; Janečková, Lucie (referee)
A testicular organoid that would sufficiently recapitulate the architecture of the testicular tissue and at the same time be able to provide the complete process of spermatogenesis in vitro has not yet been created. Achieving this goal would mean the development of a 3D model of the testis, which would mimic the in vivo situation in terms of cell arrangement and could thus contribute to a deeper understanding of the physiological functioning of the testicular microenvironment. Among other things, such a model has a great potential for clarifying the causes of male infertility and finding treatment options. This thesis dealt with the generation of organoids from mouse or human testicular cell suspensions, which can also be used, for example, to study de novo organogenesis. A total of four 3D culture systems were tested, of which the soft agar culture system (SACS) achieved the best results. Furthermore, as part of this thesis, the procedure for preparing testicular organoids for light sheet microscopy was successfully introduced and optimized, enabling the evaluation of their internal structure. From the testicular cell suspension of a 5-day-old mouse, it was possible to prepare testicular organoids, the structure of which in some respects resembled the organization of the testis in vivo. The...
|
|
|
Bioinformatical analysis of the complex multidimensional microscopy datasets
Backová, Lenka ; Černý, Jan (advisor) ; Čapek, Martin (referee)
Microscopy is embedded in the history of life sciences and vice versa. Recent advances in the field present new challenges as new revolutionary technologies arise. Sample prepa- ration, microscope operation and data analysis have become particularly demanding re- quiring specific interdisciplinary expertise. Bioimaging data analysis is computationally demanding, as microscopy technologies can easily acquire data of exceptional size, often in terabytes. Correct analysis requires computer vision knowledge, as well as knowledge of studied biological systems and last, but not least deep understanding of microscopy technology. Tools available for the analysis of the imaging data vary from open-source customizable software with a coverage of multiple tasks to a task specific proprietary software. To choose the best tools for the analysis, analysts should know their options and tasks at hand. In bioimage analysis the tasks needed to be employed depend on the desired outcome and the acquisition technology. Amongst the possible tasks to con- sider belong deconvolution, segmentation and registration. Amount of approaches and algorithms available is progressively growing, resulting in a complex field, difficult to be easily familiar with. My thesis covers different microscopy technologies with emphasis on...
|
guest ::
